The objective of this study is to investigate musculoskeletal and neuro-mechanical adaptations to frequent wearable device use. Probing impacts of both unloading and behavioral changes can give insight to ‘real-world’ adaption and feedback systems. To study structural and functional adaptations, our lab uses musculoskeletal modeling and human experiments incorporating indirect calorimetry, electromyography, ultrasonography, and 3D motion capture. Understanding effects of long-term exoskeletons use can inform user settings as well as device design and control.